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| 4种非洲猪瘟核酸检测试剂盒性能比较分析 |
| Comparison and analysis of the detection performance of four ASFV nucleic acid detection kits |
| 投稿时间：2021-02-07 |
| DOI： |
| 中文关键词: 非洲猪瘟 核酸诊断 荧光定量 敏感性 比较 |
| 英文关键词:African swine fever Nucleic acid detection qRT-PCR Sensibility Comparative |
| 基金项目: |
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| 摘要点击次数: 503 |
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| 中文摘要: |
| 目的：为猪场实验室提供科学的非洲猪瘟病毒核酸诊断试剂盒选择方案。方法：选择4种非洲猪瘟核酸检测试剂盒，通过对比梯度稀释后标准物质的线性扩增、对低含量时（20 copies/μL）的敏感性、对以纯水为样本时假阳性检出率。结果：线性扩增试验中，A试剂盒对20 copies/μL浓度的标准品检测率为100%（3/3），其他试剂盒对该浓度下的标准品，均未达到100%；低含量敏感性试验中，A试剂盒阳性检出率为91.67%（44/48），其他厂家试剂盒的检出率分别为85.42%（41/48）、79.17%（38/48）、58.33%（28/48）；假阳性试验中，A和B未出现阳性反应，而C、D试剂盒分别出现2、1个非特异性反应。结论：经过性能比较，A试剂盒更符合猪场实验室对于高敏感性和低假阳性的要求。 |
| 英文摘要: |
| Objective: To provide a scientific method for nucleic acid detection of African swine fever virus (ASFV) in pig farm diagnostic laboratory. Methods: To evaluate the four selected ASFV nucleic acid detection kits, the linear amplification assay of reference substance with serial dilution, the sensitivity assay of clinical samples in low concentration (20 copies/μL) and the detection rate of false positive in pure water samples were conducted by employing the four ASFV nucleic acid detection kits. Results: In the linear amplification test, the detection rate of kit A was 100% (3/3) at 20 copies/μL, while the detection rates of other kits were less than 100%； In the sensitivity test, the positive detection rate of kit A was 91.67% (44/48), and the positive rates of other kits were 85.42% (41/48), 79.17% (38/48) and 58.33% (28/48), respectively； In the detection of pure water samples, kits A and B showed no positive reaction, while kits C and D showed 2 and 1 reaction with nonspecific amplification, respectively. Conclusion: In comparison, the kit A with high sensitivity and low false positive rate is more suitable for application in laboratory of pig farms. |
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