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| 猪精子冷冻损伤与保护剂的研究进展 |
| Research progress on cryogenic damage and protective agent of porcine sperm |
| 投稿时间：2020-12-27 |
| DOI： |
| 中文关键词: 精液 冷冻保存 氧化损伤 影响因素 |
| 英文关键词:Semen Cryopreservation Oxidative damage Infl uencing factors |
| 基金项目:黑龙江省博士后启动基金（LBH-Q18100）；黑龙江八一农垦大学博士启动基金（XYB-2016-04）；黑龙江省大学生创新创业训练计划项目（C类国家级，项目编号：202010223044） |
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| 中文摘要: |
| 猪精液冷冻保存技术主要是利用液氮（-196℃）作为冷源，在超低温的环境下使精子生理代谢完全暂停，实现长期保存的目的。猪精子在低温环境主要损伤是细胞内结冰晶造成的机械损伤、游离的活性氧造成的化学损伤，对这两种损伤机制研究是国内外科研工作者们的研究热点。文章主要简述猪精液冷冻技术发展简况，精子在冷冻过程中受损伤机制和现阶段应用几种保护剂。 |
| 英文摘要: |
| Cryopreservation technology of porcine sperm mainly uses liquid nitrogen (-196℃) as the cold source to completely suspend the physiological metabolism of sperm in the ultra-low temperature environment, so as to achieve the purpose of long-term preservation. In low temperature environment, porcine spermatozoa is mainly damaged by ice crystals inside cells and by free reactive oxygen species. This paper mainly describes the development of pig semen Cryopreservation technology, the mechanism of sperm damage during freezing and the application of several protective agents at present. |
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