Ssc-miR-133a-5p的靶基因预测及其相关信号通路的生物信息学分析
Bioinformatics analysis of ssc-miR-133a-5p target gene prediction and related signal pathways
投稿时间:2020-09-25  
DOI:
中文关键词:  ssc-miR-133a-5p  靶基因预测  信号通路
英文关键词:Ssc-miR-133a-5p  Target gene prediction  Signal pathway
基金项目:北京市教委2019年度科技计划一般项目(KM201910020010);生猪产业技术体系北京市创新团队(BAIC02-2020)
作者单位
常雪蕊 北京农学院动物科学技术学院, 北京 102206 
赵志显 北京农学院动物科学技术学院, 北京 102206 
赵俊金 全国畜牧总站, 北京 100193 
邓晓彬 北京田园奥瑞生物科技有限公司, 北京 100096 
刘刚 全国畜牧总站, 北京 100193 
张永红 北京农学院动物科学技术学院, 北京 102206 
郭勇 北京农学院动物科学技术学院, 北京 102206 
倪和民 北京农学院动物科学技术学院, 北京 102206 
王楚端 中国农业大学动物科学技术学院, 北京 100194 
邢凯 北京农学院动物科学技术学院, 北京 102206 
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中文摘要:
      课题组前期通过高通量测序技术发现,microRNAssc-miR-133a-5p在肥胖猪脂肪组织中表达水平显著下调,推测其可能在脂肪沉积过程中发挥了重要作用。分析ssc-miR-133a-5p序列保守性,利用TargetScan,miRDB和miRWalk在线分析工具预测其候选靶基因,进一步对候选靶基因进行蛋白质互作分析以及KEGG分析,最后将预测的靶基因与课题组前期筛选出的与猪脂肪沉积能力相关的基因取交集。结果表明ssc-miR-133a-5p候选靶基因PPARGC1A与RORA等,参与AMPK,TNF,与胰岛素分泌等信号通路。结果提示ssc-miR-133a-5p的候选靶基因可能通过多种信号通路发挥重要作用。文章的结果可为microRNA参与猪的脂肪生成调节机制提供科学依据。
英文摘要:
      In the early stage of our research group, through high-throughput sequencing technology, it was found that the expression level of microRNAssc-miR-133a-5p in the fat tissue of obese pigs was signifi cantly down-regulated. It is speculated that it may play an important role in the process of fat deposition. We Analyzed the conservation of ssc-miR-133a-5p sequence, used TargetScan, miRDB and miRWalk online analysis tools to predict its candidate target genes, and further analyzed candidate target genes for protein interaction and KEGG pathway analysis, and fi nally predicted the target genes intersection with genes related to pig fat deposition ability screened by the research group in the early stage. The results show that ssc-miR-133a-5p candidate target genes PPARGC1A and RORA are involved in signal pathways such as AMPK, TNF, and insulin secretion. The results suggest that candidate target genes of ssc-miR-133a-5p may play an important role through multiple signaling pathways.The results of this study can provide a scientific basis for microRNA to participate in the regulation of pig adipogenesis.
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