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| 猪0.5 mL细管冻精不同解冻方法精子质量的研究 |
| Study on the quality of sperm by different thawing methods of 0.5ml straw frozen boar semen |
| 投稿时间：2020-05-26 |
| DOI： |
| 中文关键词: 计算机辅助精子分析系统 冷冻精液 解冻方法 公猪 Topsis |
| 英文关键词:CASA Frozen semen Thawing procedure Boar Topsis |
| 基金项目:北京市农业农村局2020年农业科技项目（任务编号20200104）、2020生猪产业技术体系北京市创新团队天鹏兴旺养猪技术综合试验站工作经费支持 |
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| 中文摘要: |
| 利用计算机辅助精子分析系统（CASA）结合顶体完整率（NAR）的检查，研究了长白猪0.5 mL细管冻精4种解冻程序后精液的质量，综合评价不同解冻方法。结果表明：1）精子冷冻-解冻后在曲线速度（VCL）、直线速度（VSL）、平均路径速度（VAP）、直线性（LIN）、前向性（STR）、鞭打频率（BCF）以及A+B类精子比例的检测性状上降低，差异极显著（P<0.01）；精子头侧摆幅度（ALH）也显著减小（P<0.05），活率（Motile rate）变化差异不显著（P>0.05）； 2）四组解冻程序中，38℃ 20 s解冻组的活率（Motile rate，MR）、VSL、VCL、STR、A+B精子比例指标均极显著（P<0.01）优于42℃ 20 s、50℃ 15 s、62℃ 5 s解冻组，VAP、LIN与NAR差异不显著（P>0.05）； 3）Topsis综合评价的结果表明38℃ 20 s组在孵育2 h、4 h、6 h之后检测性状优于其他解冻组。由此推论，虽然解冻后精子在运动参数发生较大变化，但是38℃ 20 s的解冻效果优于其他解冻条件。 |
| 英文摘要: |
| It was studied that the quality parameters of 0.5 ml straws frozen semen from Landrace boars after 4 different thawing procedures by the computer-aided semen analysis system(CASA) combined with examinations of acrosome integrity rate(NAR), and all different thawing methods were evaluated comprehensively. The results showed that:(I) After sperm freezing and thawing, the values of VCL, VSL, VAP, Lin, STR, BCF and A+ B sperm ratio decreased significantly (p<0.01); the ALH of sperm decreased extremely significantly (p<0.05), and the motile rate decreased significantly (p<0.05) There was no significant difference in rate (p>0.05); (II) Among the four thawing groups, the motile rate, VSL, VCL, STR, a + B of 38℃ -20s thawing group were most; (III)The results of TOPSIS comprehensive evaluation showed that 38℃ -20s group was better than other thawing groups after incubation for 2 hours, 4 hours and 6 hours. It was concluded that, although the sperm motility parameters changed greatly after thawing, the thawing effect of 38℃ -20s group was better than those of other groups. |
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