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| 白藜芦醇对松辽黑猪精液冷冻的影响 |
| Effect of resveratrol on cryopreservation of Songliao black boar semen |
| 投稿时间：2020-05-15 |
| DOI： |
| 中文关键词: 公猪 精液 白藜芦醇 冷冻保存 精子质量 |
| 英文关键词:Boar Semen Resveratrol (Res) Cryopreservation Semen quality |
| 基金项目:吉林省科技发展计划项目“新吉林黑猪扩繁与产业示范化”（20170307017NY）；长春市国际科技合作计划“猪精子抗冻性分子标记的研究”（12ZX73）吉林省农业科技创新工程项目“抗氧化剂与冷冻保护剂对冷冻解冻后猪精子质量及配种成绩影响的研究”（C92072003） |
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| 中文摘要: |
| 试验旨在评价白藜芦醇（resveratrol，Res）对松辽黑猪公猪精液冷冻的影响。试验分为5组，分别为对照组（不添加Res）和Res处理组（在冷冻基础液中分别加入25 μM、50 μM、100 μM、200 μM浓度的Res）。采用手握法采集松辽黑猪精液，用5种冷冻基础液稀释，在25℃平衡1 h，17℃平衡2 h，4℃平衡3 h后灌装于0.5 mL细管中，在液氮上方3 cm处熏蒸10 min，保存在液氮罐中30 d后进行检测。样本解冻后分别检测精子活力、谷胱甘肽过氧化物酶（GSH-Px）活性、质膜完整性、过氧化氢酶（CAT）活性、顶体完整性、线粒体活性、活性氧簇（ROS）水平、DNA完整性、超氧化物歧化酶（SOD）活性以及丙二醛（MDA）水平。结果显示：与对照组相比，冷冻基础液中添加50 μM Res可以显著提高冻融后精子的活力、SOD活性、质膜完整性、CAT活性、线粒体活性、GSH-Px活性、顶体完整性（P<0.05）；可以显著降低精子ROS水平和MDA水平（P<0.05）；添加Res对DNA完整性未表现出具有显著影响（P>0.05）。结论：猪精液冷冻前添加Res可改善冻融后精子质量，添加量50μM效果最佳，但Res是否可以改善公猪冷冻精液的体外受精及人工授精能力，有待于进一步的研究。 |
| 英文摘要: |
| The purpose of this study was to evaluate the effect of resveratrol (Res) on Songliao black soar semen freezing. There were five groups in the experiment, which were Control group (do not add Res) and Res treatment group (add 25μM, 50μM, 100μM and 200μM Res to the frozen base fluid respectively). Collecting the semen of Songliao black soar by hand, diluted with five frozen base solutions, equilibrate for 1 h at 25℃, 2 h at 17℃, 3 h at 4℃, then filled in 0.5 mL straw, fumigate 3 cm above liquid nitrogen for 10 min, stored in a liquid nitrogen tank for 30 d and tested. Sperm viability, GSH-Px activity, plasma membrane integrity, CAT activity, acrosome integrity, mitochondrial activity, ROS level, DNA integrity, SOD activity and MDA level were tested after thawing. Results showed that compared with the control group, adding 50μM Res to the frozen base fluid could significantly improve the sperm viability, SOD activity, plasma membrane integrity, CAT activity, mitochondrial activity, GSH-Px activity, acrosome integrity(P<0.05); and significantly reduce the sperm ROS level and MDA level(P<0.05). The addition of Res had no significant effect on DNA integrity(P>0.05).In conclusion, adding Res to boar semen before freezing could improve the quality of sperm after freezing and thawing, the best adding concentration was 50μM. |
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